Analytical Assays For Endpoint Data Collection

At Champions Oncology, we provide cutting-edge assays tailored to meet the rigorous demands of oncology and immunology research. As one of the leading oncology and clinical specialty testing companies, we specialize in developing endpoint assays that deliver precise and reliable data, ensuring that every study we support is positioned for success. Our immuno-oncology assays are designed to push the boundaries of analytical assay development, providing critical insights that drive the next generation of oncological breakthroughs. We employ high-parameter flow cytometry to deconvolute diverse cell populations, while immunohistochemistry and plate-based tools assess tissue changes. Next Generation Sequencing and 4D Proteomics can be used to generate hypotheses that drive oncology drug strategy.

Scientific consultation throughout study design, execution, and data interpretation
Competitive turnaround times
State-of-the-art analytical laboratories

FLOW CYTOMETRY

Interrogate up to 30 parameters on each individual cell simultaneously within a sample

H&E staining of tissue using histology and immunochemistry techniques.

HISTOLOGY & IMMUNOHISTOCHEMISTRY

Assess target protein expression within a tissue of interest

Scientists pipetting into 96 well plates for Luminex or ELISA assays.

LUMINEX & ELISA

Evaluate secreted proteins such as cytokines, growth factors, and chemokines in plate-based assays

NEXT GENERATION SEQUENCING

Generate personalized medicine strategies through bioinformatic and computational analyses

4D PROTEOMICS

Utilize mass-to-charge ratio, retention time, ionic strength, and ion mobility to identify and quantify proteins

WESTERN BLOT

Assess protein expression with picogram level sensitivity in only microliters of sample

Illuminating fluorescence detecting DNA damage using the Comet Assay.

DNA DAMAGE ASSAYS

Measure activity of agents involved in DNA damage repair

IMAGING

Evaluate in vivo metastasis and ex vivo tumor infiltration

Scientist calculating Colony forming units (CFU) through a microscope.

COLONY FORMING UNIT (CFU) ASSAY

Measure clonal composition shifts in response to cytotoxic therapies

Evaluation of cell viability of tumor cells in long term culture.

CELL VIABILITY/PROLIFERATION

Evaluate the viability or proliferation of cells throughout the duration of ex vivo assays

DNA is being detected using quantitative PCR

QUANTITATIVE REAL-TIME PCR (QPCR)

Determine the absolute quantity of target DNA in each sample

Our Immuno-Oncology Assays are at the forefront of cancer research and therapeutic evaluation, offering a comprehensive assessment of cell-killing and cell infiltration capacities across various types. These assays include cytotoxicity tests, proliferation assays, and apoptosis assays, each tailored to provide detailed insights into the efficacy of immunotherapies.

By employing cutting-edge technologies and methodologies, we ensure that our assays deliver reliable and actionable data, empowering researchers to make informed decisions in their quest to combat cancer.

What is an endpoint assay?

An endpoint assay is a type of experiment used to measure a specific biological or chemical activity at a single, defined point in time. Unlike continuous or kinetic assays, which track changes over time, an endpoint assay provides a snapshot of a reaction or process once it has reached a specific point or equilibrium. These assays are commonly used in various fields, including drug development, to assess the effect of a compound by measuring a final readout, such as cell viability, enzyme activity, cell population dynamics and status,or protein and gene expression.

How do I choose the best endpoint analysis for my study?

The best endpoint analysis for your study will depend on the goal of the study and the mechanism of action of your agent. For example, you will want to measure your agent ability to kill tumor cells with a cell viability assay ex vivo or by tumor growth inhibition in vivo; additionally, you may want to use flow cytometry and imaging to measure immune cell population shifts/movement if you are testing an IO agent, or use comet assay to measure DNA damage if testing an agent that targets the DNA damage/repair mechanism, or use NGS or proteomics and Luminex/ELISA to measure changes in DNA or protein expression if you agent is expected to modify DNA/protein expression level. Our experts can help you identify the best endpoint analysis and help you plan your study efficiently and successfully.

What endpoint assays does Champions offer for clinical studies?

At Champions we can perform several assays for clinical studies: our NGS, PCR, IHC, Luminex/ELISA, and Flow Cytometry are all GCLP compliant.

What sample types are needed for endpoint assays?

The sample type needed will depend on the endpoint assay and can vary from supernatant, to dissociated cells, to tissue chunks.

Analytical Assays

A comprehensive portfolio of analytical testing assays to drive insights throughout all stages of drug development

Preclinical and Clinical Endpoint Assays & Analysis