Development and Validation of a 29-Color Immunophenotyping panel for the 3-laser Cytek Northern Lights

High-dimensional flow cytometry-based immunophenotyping is the preferred method for clinical and translational research into immunological responses. With the continued growth in the availability of fluorescent probes and advancements in cytometer technology, such as spectral flow cytometry; the ability to interrogate populations using high-complexity panels has increased exponentially. This is of interest for clinical studies where limited sample availability necessitates maximizing data output from the available analyte.

Here, we have developed a 29-color panel using a three-laser Cytek Northern LightsTM instrument on cryopreserved human PBMC samples.